Hidden Diversity in the Mountain Chorus Frog (Pseudacris brachyphona) and the Diagnosis of a New Species of Chorus Frog in Southeastern United States

Oscar E. Ospina, Lynee Tieu, Joseph J. Apodaca, and Emily Moriarty Lemmon

Copeia 108: 778–795

Supplemental Figure 1. Evanno’s ΔK and mean log-likelihood (LnL) for each assessed K during the Structure analysis. The results are shown for the complete SNP data set (A) and the data set containing a single SNP per each locus (B). In both cases, the highest ΔK suggests K = 2 as the most likely number of clusters within our dataset. Following Prunier and Holsinger (2010), we seleceted the next clustering level (K = 3) as it showed the highest mean log-likelihood.

Supplemental Figure 2. Admixture plots resulting from the Structure analysis for the complete SNP data set, and the data set containing a single SNP per each locus. Both analyses showed agreement on the sample genetic clustering.

Supplemental Figure 3. Bayesian Information Criterion (BIC) for each assessed K within the North and South Mountain Chorus frog clades. The lowest BIC was observed in both cases at K = 1, indicating that further partitioning was not supported within each clade.

Supplemental Figure 4. Response plots for mean temperature of the wettest trimester (left) and mean temperature of the driest trimester (right). The plots result from MaxEnt species distribution models based on both North and South clade occurrence records combined. The reader can notice bimodal distributions for each variable, which suggests two “optimal” temperatures and providing support to the hypothesis of ecological divergence.

Supplemental Tables

Supplemental data, parameter files, and tree file

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